NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Quantifying clustered DNA damage induction and repair by gel electrophoresis, electronic imaging and number average length analysisAssessing DNA damage induction, repair and consequences of such damages requires measurement of specific DNA lesions by methods that are independent of biological responses to such lesions. Lesions affecting one DNA strand (altered bases, abasic sites, single strand breaks (SSB)) as well as damages affecting both strands (clustered damages, double strand breaks) can be quantified by direct measurement of DNA using gel electrophoresis, gel imaging and number average length analysis. Damage frequencies as low as a few sites per gigabase pair (10(9)bp) can be quantified by this approach in about 50ng of non-radioactive DNA, and single molecule methods may allow such measurements in DNA from single cells. This review presents the theoretical basis, biochemical requirements and practical aspects of this approach, and shows examples of their applications in identification and quantitation of complex clustered damages.
Document ID
20040087471
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Sutherland, Betsy M.
(Brookhaven National Laboratory Upton, NY 11973-5000, United States)
Georgakilas, Alexandros G.
Bennett, Paula V.
Laval, Jacques
Sutherland, John C.
Gewirtz, A. M.
Date Acquired
August 21, 2013
Publication Date
October 29, 2003
Publication Information
Publication: Mutation research
Volume: 531
Issue: 2-Jan
ISSN: 0027-5107
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: CA 88335
CONTRACT_GRANT: R01 CA 86897
Distribution Limits
Public
Copyright
Other
Keywords
NASA Program Biological Systems Research
NASA Discipline Radiation Health
Review
Review, Academic
Non-NASA Center

Available Downloads

There are no available downloads for this record.
No Preview Available