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Induction of genomic instability in TK6 human lymphoblasts exposed to 137Cs gamma radiation: comparison to the induction by exposure to accelerated 56Fe particlesThe induction of genomic instability in TK6 human lymphoblasts by exposure to (137)Cs gamma radiation was investigated by measuring the frequency and characteristics of unstable clones isolated approximately 36 generations after exposure. Clones surviving irradiation and control clones were analyzed for 17 characteristics including chromosomal aberrations, growth defects, alterations in response to a second irradiation, and mutant frequencies at the thymidine kinase and Na(+)/K(+) ATPase loci. Putative unstable clones were defined as those that exhibited a significant alteration in one or more characteristics compared to the controls. The frequency and characteristics of the unstable clones were compared in clones exposed to (137)Cs gamma rays or (56)Fe particles. The majority of the unstable clones isolated after exposure to either gamma rays or (56)Fe particles exhibited chromosomal instability. Alterations in growth characteristics, radiation response and mutant frequencies occurred much less often than cytogenetic alterations in these unstable clones. The frequency and complexity of the unstable clones were greater after exposure to (56)Fe particles than to gamma rays. Unstable clones that survived 36 generations after exposure to gamma rays exhibited increases in the incidence of dicentric chromosomes but not of chromatid breaks, whereas unstable clones that survived 36 generations after exposure to (56)Fe particles exhibited increases in both chromatid and chromosome aberrations.
Document ID
20040087689
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Evans, Helen H.
(Case Western Reserve University Cleveland, Ohio 44106-4942, United States)
Horng, Min-Fen
Ricanati, Marlene
Diaz-Insua, M.
Jordan, Robert
Schwartz, Jeffrey L.
Date Acquired
August 21, 2013
Publication Date
June 1, 2003
Publication Information
Publication: Radiation research
Volume: 159
Issue: 6
ISSN: 0033-7587
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: CA73931
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Radiation Health

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