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Glucose-dependent insulinotropic peptide stimulates thymidine incorporation in endothelial cells: role of endothelin-1We have previously characterized the receptor for glucose-dependent insulinotropic polypeptide (GIPR) in vascular endothelial cells (EC). Different EC types were found to contain distinct GIPR splice variants. To determine whether activation of the GIPR splice variants resulted in different cellular responses, we examined GIP effects on human umbilical vein endothelial cells (HUVEC), which contain two GIPR splice variants, and compared them with a spontaneously transformed human umbilical vein EC line, ECV 304, which contains four GIPR splice variants. GIP dose-dependently stimulated HUVEC and ECV 304 proliferation as measured by [3H]thymidine incorporation. GIP increased endothelin-1 (ET-1) secretion from HUVEC but not from ECV 304. Use of the endothelin B receptor blocker BQ-788 resulted in an inhibition of [3H]thymidine incorporation in HUVEC but not in ECV 304. These findings suggest that, although GIP increases [3H]thymidine incorporation in both HUVEC and ECV 304, this proliferative response is mediated by ET-1 only in HUVEC. These differences in cellular response to GIP may be related to differences in activation of GIPR splice variants.
Document ID
20040087717
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Ding, Ke-Hong
(Institute of Molecular Medicine and Genetics, Medical College of Georgia Dept. of Medicine, 120 15th St., Augusta, GA 30912, United States)
Zhong, Qing
Isales, Carlos M.
Iscules, C. M.
Date Acquired
August 21, 2013
Publication Date
August 1, 2003
Publication Information
Publication: American journal of physiology. Endocrinology and metabolism
Volume: 285
Issue: 2
ISSN: 0193-1849
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Clinical Medicine
Non-NASA Center

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