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Development of a nanostructured DNA delivery scaffold via electrospinning of PLGA and PLA-PEG block copolymersThe present work utilizes electrospinning to fabricate synthetic polymer/DNA composite scaffolds for therapeutic application in gene delivery for tissue engineering. The scaffolds are non-woven, nano-fibered, membranous structures composed predominantly of poly(lactide-co-glycolide) (PLGA) random copolymer and a poly(D,L-lactide)-poly(ethylene glycol) (PLA-PEG) block copolymer. Release of plasmid DNA from the scaffolds was sustained over a 20-day study period, with maximum release occurring at approximately 2 h. Cumulative release profiles indicated amounts released were approximately 68-80% of the initially loaded DNA. Variations in the PLGA to PLA-PEG block copolymer ratio vastly affected the overall structural morphology, as well as both the rate and efficiency of DNA release. Results indicated that DNA released directly from these electrospun scaffolds was indeed intact, capable of cellular transfection, and successfully encoded the protein beta-galactosidase. When tested under tensile loads, the electrospun polymer/DNA composite scaffolds exhibited tensile moduli of approximately 35 MPa, with approximately 45% strain initially. These values approximate those of skin and cartilage. Taken together, this work represents the first successful demonstration of plasmid DNA incorporation into a polymer scaffold using electrospinning.
Document ID
20040087724
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Luu, Y. K.
(State University of New York at Stony Brook Stony Brook, NY 11794-2580, United States)
Kim, K.
Hsiao, B. S.
Chu, B.
Hadjiargyrou, M.
Hadjiargyou, M.
Date Acquired
August 21, 2013
Publication Date
April 29, 2003
Publication Information
Publication: Journal of controlled release : official journal of the Controlled Release Society
Volume: 89
Issue: 2
ISSN: 0168-3659
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: GM 63283-02
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Program Fundamental Space Biology
NASA Discipline Cell Biology

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