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Synchrony in human, mouse and bacterial cell cultures--a comparisonGrowth characteristics of synchronous human MOLT-4, human U-937 and mouse L1210 cultures produced with a new minimally-disturbing technology were compared to each other and to synchronous Escherichia coli B/r. Based on measurements of cell concentrations during synchronous growth, synchrony persisted in similar fashion for all cells. Cell size and DNA distributions in the mammalian cultures also progressed synchronously and reproducibly for multiple cell cycles. The results demonstrate that unambiguous multi-cycle synchrony, critical for verifying the absence of significant growth imbalances induced by the synchronization procedure, is feasible with these cell lines, and possibly others.
Document ID
20040087744
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Helmstetter, Charles E.
(Florida Institute of Technology Melbourne, FL 32901, United States)
Thornton, Maureen
Romero, Ana
Eward, K. Leigh
Date Acquired
August 21, 2013
Publication Date
January 1, 2003
Publication Information
Publication: Cell cycle (Georgetown, Tex.)
Volume: 2
Issue: 1
ISSN: 1538-4101
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Cell Biology

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