NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Instrumentation and methodology for quantifying GFP fluorescence in intact plant organsThe General Fluorescence Plant Meter (GFP-Meter) is a portable spectrofluorometer that utilizes a fiber-optic cable and a leaf clip to gather spectrofluorescence data. In contrast to traditional analytical systems, this instrument allows for the rapid detection and fluorescence measurement of proteins under field conditions with no damage to plant tissue. Here we discuss the methodology of gathering and standardizing spectrofluorescence data from tobacco and canola plants expressing GFP. Furthermore, we demonstrate the accuracy and effectiveness of the GFP-Meter. We first compared GFP fluorescence measurements taken by the GFP-Meter to those taken by a standard laboratory-based spectrofluorometer, the FluoroMax-2. Spectrofluorescence measurements were taken from the same location on intact leaves. When these measurements were tested by simple linear regression analysis, we found that there was a positive functional relationship between instruments. Finally, to exhibit that the GFP-Meter recorded accurate measurements over a span of time, we completed a time-course analysis of GFP fluorescence measurements. We found that only initial measurements were accurate; however, subsequent measurements could be used for qualitative purposes.
Document ID
20040087771
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Millwood, R. J.
(UNC Greensboro, NC, United States)
Halfhill, M. D.
Harkins, D.
Russotti, R.
Stewart, C. N. Jr
Date Acquired
August 21, 2013
Publication Date
March 1, 2003
Publication Information
Publication: BioTechniques
Volume: 34
Issue: 3
ISSN: 0736-6205
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Evaluation Studies
Validation Studies
NASA Discipline Life Sciences Technologies
Non-NASA Center

Available Downloads

There are no available downloads for this record.
No Preview Available