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Control of T lymphocyte morphology by the GTPase RhoBACKGROUND: Rho family GTPase regulation of the actin cytoskeleton governs a variety of cell responses. In this report, we have analyzed the role of the GTPase Rho in maintenance of the T lymphocyte actin cytoskeleton. RESULTS: Inactivation of the GTPase Rho in the human T lymphocytic cell line HPB-ALL does not inhibit constitutively high adhesion to the integrin beta1 substrate fibronectin. It did however result in the aberrant extension of finger-like dendritic processes on the substrates VCAM-1, Fn, and mAb specific to beta1 integrins. Time-lapse video microscopy demonstrated that C3 induced extensions were primarily the result of an altered pseudopod elongation rather than retraction. Once the stellate pseudopodia extended, none retracted, and cells became completely immobile. Filipodial structures were absent and the dendritic-like processes in C3 treated cells were rich in filamentous actin. Immunolocalization of RhoA in untreated HPB-ALL cells spreading on fibronectin demonstrated a diffuse staining pattern within the pseudopodia. In C3 treated cells, clusters of RhoA were pronounced and localized within the altered extensions. CONCLUSIONS: GTPase Rho is actively involved in the regulation of T lymphocyte morphology and motility.
Document ID
20040087822
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Woodside, Darren G.
(University of Texas M, Anderson Cancer Center D, Houston 77030, United States)
Wooten, David K.
Teague, T. Kent
Miyamoto, Yuko J.
Caudell, Eva G.
Udagawa, Taturo
Andruss, Bernard F.
McIntyre, Bradley W.
Date Acquired
August 21, 2013
Publication Date
February 24, 2003
Publication Information
Publication: BMC cell biology [electronic resource]
Volume: 4
Issue: 1
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: CA62596
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Cell Biology

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