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Enhancement of anion-exchange chromatography of DNA using compaction agentsThe use of adsorptive chromatography for preparative nucleic acid separations is often limited by low capacity. The possibility that the adsorbent surface area sterically accessible to nucleic acid molecules could be increased by reducing their radius of gyration with compaction agents has been investigated. The equilibrium adsorption capacity of Q Sepharose anion-exchange matrix for plasmid DNA at 600 mM NaCl was enhanced by up to ca. 40% in the presence of 2.5 mM spermine. In addition, compaction agent selectivity has been demonstrated. Spermine, for example, enhances the adsorption of both plasmid and genomic DNA, spermidine enhances binding only of plasmid, and hexamine cobalt enhances only the binding of genomic DNA. Compaction may be generally useful for enhancing adsorptive separations of nucleic acids.
Document ID
20040087869
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Murphy, Jason C.
(University of Houston 4800 Calhoun Ave., Houston, TX 77004-4004, United States)
Fox, George E.
Willson, Richard C.
Date Acquired
August 21, 2013
Publication Date
January 17, 2003
Publication Information
Publication: Journal of chromatography. A
Volume: 984
Issue: 2
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Environmental Health
Non-NASA Center

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