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Activation of L-type calcium channels is required for gap junction-mediated intercellular calcium signaling in osteoblastic cellsThe propagation of mechanically induced intercellular calcium waves (ICW) among osteoblastic cells occurs both by activation of P2Y (purinergic) receptors by extracellular nucleotides, resulting in "fast" ICW, and by gap junctional communication in cells that express connexin43 (Cx43), resulting in "slow" ICW. Human osteoblastic cells transmit intercellular calcium signals by both of these mechanisms. In the current studies we have examined the mechanism of slow gap junction-dependent ICW in osteoblastic cells. In ROS rat osteoblastic cells, gap junction-dependent ICW were inhibited by removal of extracellular calcium, plasma membrane depolarization by high extracellular potassium, and the L-type voltage-operated calcium channel inhibitor, nifedipine. In contrast, all these treatments enhanced the spread of P2 receptor-mediated ICW in UMR rat osteoblastic cells. Using UMR cells transfected to express Cx43 (UMR/Cx43) we confirmed that nifedipine sensitivity of ICW required Cx43 expression. In human osteoblastic cells, gap junction-dependent ICW also required activation of L-type calcium channels and influx of extracellular calcium.
Document ID
20040087978
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Jorgensen, Niklas Rye
(Copenhagen University Hospitals, Copenhagen Hospital Corporation DK-2650 Hvidovre Denmark)
Teilmann, Stefan Cuoni
Henriksen, Zanne
Civitelli, Roberto
Sorensen, Ole Helmer
Steinberg, Thomas H.
Date Acquired
August 21, 2013
Publication Date
February 7, 2003
Publication Information
Publication: The Journal of biological chemistry
Volume: 278
Issue: 6
ISSN: 0021-9258
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Cell Biology

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