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Karyotypes of Human Lymphocytes Exposed to High-Energy Iron IonsChromosomal aberrations were analyzed using multicolor fluorescence in situ hybridization (mFISH) in human peripheral blood lymphocytes after in vitro exposure to gamma rays or accelerated (56)Fe ions (1 GeV/nucleon, 145 keV/microm) at Brookhaven National Laboratory (Upton, NY). Doses of 0.3 and 3 Gy were used for both radiation types. Chromosomes were prematurely condensed by a phosphatase inhibitor (calyculin A) to avoid the population selection bias observed at metaphase as a result of the severe cell cycle delays induced by heavy ions. A total of 1053 karyotypes (G(2) and M phases) were analyzed in irradiated lymphocytes. Results revealed different distribution patterns for chromosomal aberrations after low- and high-LET radiation exposures: Heavy ions induced a much higher fraction of cells with multiple aberrations, while the majority of the aberrant cells induced by low doses of gamma rays contained a single aberration. The high fraction of complex-type exchanges after heavy ions leads to an overestimation of simple-type asymmetrical interchanges (dicentrics) from analysis of Giemsa-stained samples. However, even after a dose of 3 Gy iron ions, about 30% of the cells presented no complex-type exchanges. The involvement of individual chromosomes in exchanges was similar for densely and sparsely ionizing radiation, and no statistically significant evidence of a nonrandom involvement of specific chromosomes was detected.
Document ID
20040088034
Acquisition Source
Johnson Space Center
Document Type
Reprint (Version printed in journal)
Authors
Durante, M.
(Universita Federico II Napoli, Italy)
George, K.
Wu, H.
Cucinotta, F. A.
Date Acquired
August 21, 2013
Publication Date
November 1, 2002
Publication Information
Publication: Radiation research
Volume: 158
Issue: 5
ISSN: 0033-7587
Subject Category
Life Sciences (General)
Report/Patent Number
JSC-CN-7478
Distribution Limits
Public
Copyright
Other
Keywords
NASA Center JSC
NASA Discipline Radiation Health

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