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Mechanical stimulation improves tissue-engineered human skeletal muscleHuman bioartificial muscles (HBAMs) are tissue engineered by suspending muscle cells in collagen/MATRIGEL, casting in a silicone mold containing end attachment sites, and allowing the cells to differentiate for 8 to 16 days. The resulting HBAMs are representative of skeletal muscle in that they contain parallel arrays of postmitotic myofibers; however, they differ in many other morphological characteristics. To engineer improved HBAMs, i.e., more in vivo-like, we developed Mechanical Cell Stimulator (MCS) hardware to apply in vivo-like forces directly to the engineered tissue. A sensitive force transducer attached to the HBAM measured real-time, internally generated, as well as externally applied, forces. The muscle cells generated increasing internal forces during formation which were inhibitable with a cytoskeleton depolymerizer. Repetitive stretch/relaxation for 8 days increased the HBAM elasticity two- to threefold, mean myofiber diameter 12%, and myofiber area percent 40%. This system allows engineering of improved skeletal muscle analogs as well as a nondestructive method to determine passive force and viscoelastic properties of the resulting tissue.
Document ID
20040088051
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Powell, Courtney A.
(Brown University Providence, Rhode Island 02912, United States)
Smiley, Beth L.
Mills, John
Vandenburgh, Herman H.
Date Acquired
August 21, 2013
Publication Date
November 1, 2002
Publication Information
Publication: American journal of physiology. Cell physiology
Volume: 283
Issue: 5
ISSN: 0363-6143
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: R01-AG-15415
CONTRACT_GRANT: R44-AG-14958
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Musculoskeletal

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