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Regional alterations of type I collagen in rat tibia induced by skeletal unloadingSkeletal unloading induces loss of mineral density in weight-bearing bones that leads to inferior bone mechanical strength. This appears to be caused by a failure of bone formation; however, its mechanisms still are not well understood. The objective of this study was to characterize collagen, the predominant matrix protein in bone, in various regions of tibia of rats that were subjected to skeletal unloading by 4 weeks tail suspension. Sixteen male Sprague-Dawley rats (4 months old) were divided into tail suspension and ambulatory controls (eight rats each). After the tail suspension, tibias from each animal were collected and divided into five regions and collagen was analyzed. The collagen cross-linking and the extent of lysine (Lys) hydroxylation in unloaded bones were significantly altered in proximal epiphysis, diaphysis, and, in particular, proximal metaphysis but not in distal regions. The pool of immature/nonmineralized collagen measured by its extractability with a chaotropic solvent was significantly increased in proximal metaphysis. These results suggest that skeletal unloading induced an accumulation of post-translationally altered nonmineralized collagen and that these changes are bone region specific. These alterations might be caused by impaired osteoblastic function/differentiation resulting in a mineralization defect.
Document ID
20040088144
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Shiiba, Masashi
(Dental Research Center, University of North Carolina at Chapel Hill 27599, United States)
Arnaud, Sara B.
Tanzawa, Hideki
Kitamura, Eiji
Yamauchi, Mitsuo
Date Acquired
August 21, 2013
Publication Date
September 1, 2002
Publication Information
Publication: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Volume: 17
Issue: 9
ISSN: 0884-0431
Subject Category
Aerospace Medicine
Funding Number(s)
CONTRACT_GRANT: DE10489
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Cell Biology

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