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Regulation of period 1 expression in cultured rat pinealThe aim of the present study was to investigate the in vitro expression of Period 1 (Per1), Period 2 (Per2) and arylalkylamine N-acetyltransferase (AA-NAT) genes in the rat pineal gland to understand the mechanism(s) regulating the expression of these genes in this organ. Pineals, when maintained in vitro for 5 days, did not show circadian rhythmicity in the expression of any of the three genes monitored. Norepinephrine (NE) induced AA-NAT and Per1, whereas its effect on Per2 was negligible. Contrary to what was observed in other systems, NE stimulation did not induce circadian expression of Per1. The effect of NE on Per1 level was dose- and receptor subtype-dependent, and both cAMP and cGMP induced Per1. Per1 was not induced by repeated NE - or forskolin - stimulation. Protein synthesis was not necessary for NE-induced Per1, but it was for reduction of Per1 following NE stimulation. Per1 transcription in pinealocytes was activated by BMAL1/CLOCK. Our results indicate that important differences are present in the regulation of these genes in the mammalian pineal. Copyright 2002 S. Karger AG, Basel.
Document ID
20040088259
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Fukuhara, Chiaki
(Neuroscience Institute, Morehouse School of Medicine Atlanta, GA 30310-1495, United States)
Dirden, James C.
Tosini, Gianluca
Date Acquired
August 21, 2013
Publication Date
March 1, 2002
Publication Information
Publication: Neuro-Signals
Volume: 11
Issue: 2
ISSN: 1424-862X
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NS 34194
CONTRACT_GRANT: NS 38483
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Space Human Factors
Non-NASA Center

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