NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Effects of microgravity on myogenic factor expressions during postnatal development of rat skeletal muscleTo clarify the role of gravity in the postnatal development of skeletal muscle, we exposed neonatal rats at 7 days of age to microgravity. After 16 days of spaceflight, tibialis anterior, plantaris, medial gastrocnemius, and soleus muscles were removed from the hindlimb musculature and examined for the expression of MyoD-family transcription factors such as MyoD, myogenin, and MRF4. For this purpose, we established a unique semiquantitative method, based on RT-PCR, using specific primers tagged with infrared fluorescence. The relative expression of MyoD in the tibialis anterior and plantaris muscles and that of myogenin in the plantaris and soleus muscles were significantly reduced (P < 0.001) in the flight animals. In contrast, MRF4 expression was not changed in any muscle. These results suggest that MyoD and myogenin, but not MRF4, are sensitive to gravity-related stimuli in some skeletal muscles during postnatal development.
Document ID
20040088372
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Inobe, Manabu
(National Institute of Neuroscience, National Center of Neurology and Psychiatry Kodaira, Tokyo 187-8502, Japan)
Inobe, Ikuko
Adams, Gregory R.
Baldwin, Kenneth M.
Takeda, Shin'Ichi
Date Acquired
August 21, 2013
Publication Date
May 1, 2002
Publication Information
Publication: Journal of applied physiology (Bethesda, Md. : 1985)
Volume: 92
Issue: 5
ISSN: 8750-7587
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
STS-90 Shuttle Project
Flight Experiment
NASA Discipline Musculoskeletal
manned
short duration
NASA Experiment Number 9301103

Available Downloads

There are no available downloads for this record.
No Preview Available