NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Clustered DNA damages induced by high and low LET radiation, including heavy ionsClustered DNA damages--here defined as two or more lesions (strand breaks, oxidized purines, oxidized pyrimidines or abasic sites) within a few helical turns--have been postulated as difficult to repair accurately, and thus highly significant biological lesions. Further, attempted repair of clusters may produce double strand breaks (DSBs). However, until recently, there was no way to measure ionizing radiation-induced clustered damages, except DSB. We recently described an approach for measuring classes of clustered damages (oxidized purine clusters, oxidized pyrimidine clusters, abasic clusters, along with DSB). We showed that ionizing radiation (gamma rays and Fe ions, 1 GeV/amu) does induce such clusters in genomic DNA in solution and in human cells. These studies also showed that each damage cluster results from one radiation hit (and its track), thus indicating that they can be induced by very low doses of radiation, i.e. two independent hits are not required for cluster induction. Further, among all complex damages, double strand breaks comprise--at most-- ~20%, with the other clustered damages being at least 80%.
Document ID
20040088539
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Sutherland, B. M.
(Brookhaven National Laboratory Upton, NY 11973, United States)
Bennett, P. V.
Schenk, H.
Sidorkina, O.
Laval, J.
Trunk, J.
Monteleone, D.
Sutherland, J.
Lowenstein, D. I.
Date Acquired
August 21, 2013
Publication Date
January 1, 2001
Publication Information
Publication: Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB)
Volume: 17 Suppl 1
ISSN: 1120-1797
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Radiation Health
Non-NASA Center

Available Downloads

There are no available downloads for this record.
No Preview Available