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Nonenzymatic synthesis of RNA and DNA oligomers on hexitol nucleic acid templates: the importance of the A structureHexitol nucleic acid (HNA) is an analogue of DNA containing the standard nucleoside bases, but with a phosphorylated 1,5-anhydrohexitol backbone. HNA oligomers form duplexes having the nucleic acid A structure with complementary DNA or RNA oligomers. The HNA decacytidylate oligomer is an efficient template for the oligomerization of the 5'-phosphoroimidazolides of guanosine or deoxyguanosine. Comparison of the oligomerization efficiencies on HNA, RNA, and DNA decacytidylate templates under various conditions suggests strongly that only nucleic acid double helices with the A structure support efficient template-directed synthesis when 5'-phosphoroimidazolides of nucleosides are used as substrates.
Document ID
20040088795
Acquisition Source
Goddard Space Flight Center
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Kozlov, I. A.
(Salk Institute for Biological Studies San Diego, California 92186, United States)
Politis, P. K.
Van Aerschot, A.
Busson, R.
Herdewijn, P.
Orgel, L. E.
Bada, J. L.
Dolan, M.
Date Acquired
August 21, 2013
Publication Date
March 31, 1999
Publication Information
Publication: Journal of the American Chemical Society
Volume: 121
Issue: 12
ISSN: 0002-7863
Subject Category
Exobiology
Funding Number(s)
CONTRACT_GRANT: GOA 97
CONTRACT_GRANT: NAG5-4546
CONTRACT_GRANT: NAG5-4118
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Exobiology

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