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PCR cloning and characterization of multiple ADP-glucose pyrophosphorylase cDNAs from tomatoFour ADP-glucose pyrophosphorylase (AGP) cDNAs were cloned from tomato fruit and leaves by the PCR techniques. Three of them (agp S1, agp S2, and agp S3) encode the large subunit of AGP, the fourth one (agp B) encodes the small subunit. The deduced amino acid sequences of the cDNAs show very high identities (96-98%) to the corresponding potato AGP isoforms, although there are major differences in tissue expression profiles. All four tomato AGP transcripts were detected in fruit and leaves; the predominant ones in fruit are agp B and agp S1, whereas in leaves they are agp B and agp S3. Genomic southern analysis suggests that the four AGP transcripts are encoded by distinct genes.
Document ID
20040088873
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Chen, B. Y.
(The State University of New Jersey New Brunswick 08901-8520, United States)
Janes, H. W.
Gianfagna, T.
Date Acquired
August 21, 2013
Publication Date
August 7, 1998
Publication Information
Publication: Plant science (Shannon, Ireland)
Volume: 136
Issue: 1
ISSN: 0168-9452
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Life Support Systems

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