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Multiplex titration RT-PCR: rapid determination of gene expression patterns for a large number of genesWe have developed an improved method for determination of gene expression levels with RT-PCR. The procedure is rapid and does not require extensive optimization or densitometric analysis. Since the detection of individual transcripts is PCR-based, small amounts of tissue samples are sufficient for the analysis of expression patterns in large gene families. Using this method, we were able to rapidly screen nine members of the Aux/IAA family of auxin-responsive genes and identify those genes which vary in message abundance in a tissue- and light-specific manner. While not offering the accuracy of conventional semi-quantitative or competitive RT-PCR, our method allows quick screening of large numbers of genes in a wide range of RNA samples with just a thermal cycler and standard gel analysis equipment.
Document ID
20040088976
Acquisition Source
Goddard Space Flight Center
Document Type
Reprint (Version printed in journal)
Authors
Nebenfuhr, A.
(Oregon State University Corvallis 97331-2902, United States)
Lomax, T. L.
Date Acquired
August 21, 2013
Publication Date
January 1, 1998
Publication Information
Publication: Plant molecular biology reporter / ISPMB
Volume: 16
ISSN: 0735-9640
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAG5-6373
CONTRACT_GRANT: IBN9-423651
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Plant Biology

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