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Immunological and biochemical evidence for nuclear localization of annexin in peasImmunofluorescent localization of annexins using an anti-pea annexin polyclonal antibody (anti-p35) in pea (Pisum sativum) leaf and stem epidermal peels showed staining of the nuclei and the cell periphery. Nuclear staining was also seen in cell teases prepared from pea plumules. The amount of nuclear stain was reduced both by fixation time and by dehydration and organic solvent treatment. Observation with confocal microscopy demonstrated that the anti-p35 stain was diffusely distributed throughout the nuclear structure. Immunoblots of purified nuclei, nuclear envelope matrix, nucleolar, and chromatin fractions showed a cross-reactive protein band of 35 kDa. These data are the first to show annexins localized in plant cell nuclei where they may play a role in nuclear function.
Document ID
20040088994
Acquisition Source
Headquarters
Document Type
Reprint (Version printed in journal)
Authors
Clark, G. B.
(University of Texas Austin 78713, United States)
Dauwalder, M.
Roux, S. J.
Date Acquired
August 21, 2013
Publication Date
September 1, 1998
Publication Information
Publication: Plant physiology and biochemistry : PPB / Societe francaise de physiologie vegetale
Volume: 36
Issue: 9
ISSN: 0981-9428
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAGW-1519
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Plant Biology

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