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Organization of photosystem I polypeptides examined by chemical cross-linkingPhotosystem I from the cyanobacterium Synechocystis sp. PCC 6803 was examined using the chemical cross-linkers glutaraldehyde and N-ethyl-1-3-[3-(dimethylamino)propyl]carbodiimide to investigate the organization of the polypeptide subunits. Thylakoid membranes and photosystem I, which was isolated by Triton X-100 fractionation, were treated with cross-linking reagents and were resolved using a Tricine/urea low-molecular-weight resolution gel system. Subunit-specific antibodies and western blotting analysis were used to identify the components of cross-linked species. These analyses identified glutaraldehyde-dependent cross-linking products composed of small amounts of PsaD and PsaC, PsaC and PsaE, and PsaE and PsaF. The novel cross-link between PsaE and PsaF was also observed following treatment with N-ethyl-1-3-[3-(dimethylamino)propyl]carbodiimide. These cross-linking results suggest a structural interaction between PsaE and PsaF and predict a transmembrane topology for PsaF.
Document ID
20040089317
Acquisition Source
Kennedy Space Center
Document Type
Reprint (Version printed in journal)
Authors
Armbrust, T. S.
(Kansas State University Manhattan 66506-4901, United States)
Chitnis, P. R.
Guikema, J. A.
Spooner, B. S.
Date Acquired
August 21, 2013
Publication Date
January 1, 1996
Publication Information
Publication: Plant physiology
Volume: 111
ISSN: 0032-0889
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAG10-0142
CONTRACT_GRANT: 9337306-9147
CONTRACT_GRANT: NAGW-2328
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Plant Biology
NASA Discipline Cell Biology
Non-NASA Center

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