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Ethylene-associated phase change from juvenile to mature phenotype of daylily (Hemerocallis) in vitroHemerocallis plantlets maintained in vitro for extended periods of time in tightly closed culture vessels frequently show a phenotype, albeit on a miniaturized scale, typical of more mature, field-grown plants. The positive relationship of elevated ethylene in the headspace of such vessels to the phase shift from juvenile to mature form is established. Rigorous restriction in air exchange with the external environment by means of silicone grease seals hastens the phase change and improves uniformity of response. Although some plantlets may take longer to accumulate enough ethylene in sealed jars to undergo change, added ethylene and ethylene-releasing agents promote it. Ethylene adsorbants (e.g. mercuric perchlorate) block the shift of juvenile to mature form. Critical ambient ethylene level for the shift is ca 1 microliter l-1. Levels up to 1000 microliters l-1 do not hasten the response but are not toxic. The phase change is fully reversible when air exchange permits ethylene to drop below 1 microliter l-1. At least 1 microliter l-1 ethylene is required to sustain the mature phenotype. The ethylene synthesis inhibitor aminoethoxyvinylglycine (AVG) prevents the phase change, while the ethylene biosynthesis intermediate 1-aminocyclopropanecarboxylic acid (ACC) improves it. KOH, as a CO2 absorbent, does not prevent the phase change. Histology sections demonstrate subtle changes in the form of shoot tips of plantlets undergoing phase change.
Document ID
20040089911
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Smith, D. L.
(State Univ. of New York at Stony Brook 11794-5215 United States)
Kelly, K.
Krikorian, A. D.
Date Acquired
August 21, 2013
Publication Date
January 1, 1989
Publication Information
Publication: Physiologia plantarum
Volume: 76
ISSN: 0031-9317
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Plant Biology
NASA Program Space Biology
NASA Discipline Number 29-20
Non-NASA Center

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