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Comparison of axillary bud growth and patatin accumulation in potato leaf cuttings as assays for tuber inductionSingle-node leaf cuttings from potatoes (Solanum tuberosum L.) cvs. Norland, Superior, Norchip, and Kennebec, were used to assess tuber induction in plants grown under 12, 16, and 20 h daily irradiation (400 micromol s-1 m-2 PPF). Leaf cuttings were taken from plants at four, six and 15 weeks after planting and cultured for 14 d in sand trays in humid environments. Tuber induction was determined by visually rating the type of growth at the attached axillary bud, and by measuring the accumulation of the major tuber protein, patatin, in the base of the petioles. Axillary buds from leaf cuttings of plants grown under the 12 h photoperiod consistently formed round, sessile tubers at the axils for all four cultivars at all harvests. Buds from cuttings of plants grown under the 16 and 20 h photoperiods exhibited mixed tuber, stolon, and leafy shoot growth. Patatin accumulation was highest in petioles of cuttings taken from 12 h plants for all cultivars at all harvests, with levels in 16 and 20 h cuttings approx. one-half that of the 12 h cuttings. Trends, both in visual ratings of axillary buds and in petiole patatin accumulation, followed the harvest index (ratio of tuber to total plant dry matter), suggesting that either method is an acceptable assay for tuber induction in the potato.
Document ID
20040089913
Acquisition Source
Ames Research Center
Document Type
Reprint (Version printed in journal)
Authors
Wheeler, R. M.
(University of Wisconsin Madison 53706, United States)
Hannapel, D. J.
Tibbitts, T. W.
Date Acquired
August 21, 2013
Publication Date
July 1, 1988
Publication Information
Publication: Annals of botany
Volume: 62
Issue: 1
ISSN: 0305-7364
Subject Category
Man/System Technology And Life Support
Funding Number(s)
CONTRACT_GRANT: NCC2-136
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Number 61-10
NASA Discipline Life Support Systems
Non-NASA Center
NASA Program CELSS

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