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Early events of polyoma infection: adsorption, penetration and nuclear transportPolyoma virions have different attachment proteins which are responsible for hemagglutination of erythrocytes and attachment to cultured mouse kidney cells (MKC). Virion binding studies demonstrated that MKC possess specific (productive infection) and nonspecific (nonproductive) receptors. Empty polyoma capsids have hemagglutination activity and bind to non-specific MKC receptors, but they are not capable of competing for specific virion cell receptors or preventing productive infection. Isoelectric focusing of the virion major capsid protein, VP1, separated this protein into six species (A through F). These species had identical amino acid sequences, but differed in degree of modification (phosphorylation, acetylation, sulfation and hydroxylation). Evidence based upon precipitation with specific antisera supports the view that VP1 species E is required for specific adsorption and that D and F are required for hemagglutination. The virion attachment domain has been localized to an 18 kilodalton fragment of the C-terminal region of VP1. Monopinocytotic vesicles containing 125I-labeled polyoma virions were isolated from infected MKC. A crosslinker was used to bind the MKC cell receptor(s) covalently to VP1 attachment protein, and a new 120 kilodalton band was identified by SDS-PAGE. An anti-idiotype antibody prepared against a neutralizing polyoma monoclonal antiody was used to identify a putative 50 kilodalton receptor protein from a detergent extract of MKC, as well as from MKC membrane preparation.
Document ID
20040090148
Acquisition Source
Headquarters
Document Type
Reprint (Version printed in journal)
Authors
Consigli, R. A.
(Kansas State University Manhattan 66506, United States)
Haynes, J. I. Jr
Chang, D.
Grenz, L.
Richter, D.
Spooner, B. S.
Date Acquired
August 21, 2013
Publication Date
April 1, 1992
Publication Information
Publication: Transactions of the Kansas Academy of Science
Volume: 95
Issue: 2-Jan
ISSN: 0022-8443
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: CA07319
CONTRACT_GRANT: NAGW-2328
CONTRACT_GRANT: NAGW-1197
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Developmental Biology
NASA Discipline Cell Biology
NASA Program NSCORT
NASA Discipline Number 93-10
Non-NASA Center

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