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PCR amplification of 16S rDNA from lyophilized cell cultures facilitates studies in molecular systematicsThe sequence of the major portion of a Bacillus cycloheptanicus strain SCH(T) 16S rRNA gene is reported. This sequence suggests that B. cycloheptanicus is genetically quite distinct from traditional Bacillus strains (e.g., B. subtilis) and may be properly regarded as belonging to a different genus. The sequence was determined from DNA that was produced by direct amplification of ribosomal DNA from a lyophilized cell pellet with straightforward polymerase chain reaction (PCR) procedures. By obviating the need to revive cell cultures from the lyophile pellet, this approach facilitates rapid 16S rDNA sequencing and thereby advances studies in molecular systematics.
Document ID
20040090195
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Wisotzkey, J. D.
(University of Houston Texas, United States)
Jurtshuk, P. Jr
Fox, G. E.
Date Acquired
August 21, 2013
Publication Date
January 1, 1990
Publication Information
Publication: Current microbiology
Volume: 21
ISSN: 0343-8651
Subject Category
Exobiology
Funding Number(s)
CONTRACT_GRANT: BSR8-600448
CONTRACT_GRANT: NSG-7440
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Number 52-30
NASA Discipline Exobiology
NASA Program Exobiology

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