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The role of a cell surface inhibitor in early signal transduction associated with the regulation of cell division and differentiationSerum stimulation of quiescent human fibroblast cultures resulted in a hyperphosphorylation of the nuclear retinoblastoma gene susceptibility product (RB). However, serum stimulation in the presence of 9 x 10(-8) M of a purified bovine sialoglycopeptide (SGP) cell surface inhibitor abrogated the hyperphosphorylation of the RB protein and the subsequent progression of cells through the mitotic cycle. The experimental results suggest that the SGP mediated its cell cycle arrest at a site in the cell cycle that was at the time of RB phosphorylation or somewhat upstream of the modification of this regulatory protein of cell division. Both cells serum-deprived and serum stimulated in the presence of the SGP displayed only a hypophosphorylated RB protein, consistent with the SGP-mediated cell cycle arrest point being near the G1/S interface.
Document ID
20040090247
Acquisition Source
Headquarters
Document Type
Reprint (Version printed in journal)
Authors
Johnson, T. C.
(Kansas State University Manhattan 66506-4903, United States)
Enebo, D. J.
Moos, P. J.
Fattaey, H. K.
Spooner, B. S.
Date Acquired
August 21, 2013
Publication Date
January 1, 1992
Publication Information
Publication: Transactions of the Kansas Academy of Science
Volume: 95
Issue: 2-Jan
ISSN: 0022-8443
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAGW-1197
CONTRACT_GRANT: NAGW-2328
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Cell Biology
NASA Discipline Number 93-10
Non-NASA Center
NASA Discipline Developmental Biology
NASA Program NSCORT

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