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Influence of low temperature preincubation on somatic embryogenesis and ethylene emanation from orchardgrass leavesThe objectives of this study were to determine the effects of low temperature (4 degrees C) preincubation on somatic embryogenesis from orchardgrass (Dactylis glomerata L.) leaf cultures and to relate these effects to ethylene emanation during the preincubation and incubation periods. Experiments were also conducted with an ethylene biosynthesis inhibitor aminooxyacetic acid (AOA). Segments from the innermost two leaves were cultured on SH medium with 30 micromoles dicamba at 4 degrees C for 1 to 7 d before transfer to 21 degrees C. Results from a paired design showed that the embryogenic response of leaf segments preincubated at 4 degrees C was equal or superior to nonpreincubated leaves at all time periods. Ethylene emanation was decreased during the low temperature incubation. Transfer of leaf segments from 4 degrees C to 21 degrees C was accompanied by a burst of ethylene which rose to control levels within 30 min. AOA at 20 and 40 micromoles decreased ethylene emanation but did not stimulate the embryogenic response. We conclude that the stimulation of somatic embryogenesis by low temperature is probably due to factors other than suppression of ethylene biosynthesis.
Document ID
20040090254
Acquisition Source
Headquarters
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Tomaszewski, Z. Jr
(The University of Tennessee Knoxville 37901-1071)
Kuklin, A. I.
Sams, C. E.
Conger, B. V.
Date Acquired
August 21, 2013
Publication Date
January 1, 1994
Publication Information
Publication: Plant growth regulation
Volume: 14
ISSN: 0167-6903
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAGW-3141
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Number 40-50
NASA Discipline Plant Biology
NASA Program Space Biology
Non-NASA Center

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