NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Antipeptide antibodies that can distinguish specific subunit polypeptides of glutamine synthetase from bean (Phaseolus vulgaris L.)The amino acid sequences of the beta and gamma subunit polypeptides of glutamine synthetase from bean (Phaseolus vulgaris L.) root nodules are very similar. However, there are small regions within the sequences that are significantly different between the two polypeptides. The sequences between amino acids 2 and 9 and between 264 and 274 are examples. Three peptides (gamma 2-9, gamma 264-274, and beta 264-274) corresponding to these sequences were synthesized. Antibodies against these peptides were raised in rabbits and purified with corresponding peptide-Sepharose affinity chromatography. Western blot analysis of polyacrylamide gel electrophoresis of bean nodule proteins demonstrated that the anti-beta 264-274 antibodies reacted specifically with the beta polypeptide and the anti-gamma 264-274 and anti-gamma 2-9 antibodies reacted specifically with the gamma polypeptide of the native and denatured glutamine synthetase. These results showed the feasibility of using synthetic peptides in developing antibodies that are capable of distinguishing proteins with similar primary structures.
Document ID
20040090274
Acquisition Source
Headquarters
Document Type
Reprint (Version printed in journal)
Authors
Cai, X.
(Kansas State University Manhattan 66506, United States)
Henry, R. L.
Takemoto, L. J.
Guikema, J. A.
Wong, P. P.
Spooner, B. S.
Date Acquired
August 21, 2013
Publication Date
January 1, 1992
Publication Information
Publication: Plant physiology
Volume: 98
ISSN: 0032-0889
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAGW-1197
CONTRACT_GRANT: 8837120-3891
Distribution Limits
Public
Copyright
Other
Keywords
NASA Program NSCORT
NASA Discipline Cell Biology
NASA Discipline Number 93-10
Non-NASA Center

Available Downloads

There are no available downloads for this record.
No Preview Available