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Identification of amino acid sequences in the polyomavirus capsid proteins that serve as nuclear localization signalsThe molecular mechanism participating in the transport of newly synthesized proteins from the cytoplasm to the nucleus in mammalian cells is poorly understood. Recently, the nuclear localization signal sequences (NLS) of many nuclear proteins have been identified, and most have been found to be composed of a highly basic amino acid stretch. A genetic "subtractive" and a biochemical "additive" approach were used in our studies to identify the NLS's of the polyomavirus structural capsid proteins. An NLS was identified at the N-terminus (Ala1-Pro-Lys-Arg-Lys-Ser-Gly-Val-Ser-Lys-Cys11) of the major capsid protein VP1 and at the C-terminus (Glu307 -Glu-Asp-Gly-Pro-Glu-Lys-Lys-Lys-Arg-Arg-Leu318) of the VP2/VP3 minor capsid proteins.
Document ID
20040090344
Acquisition Source
Headquarters
Document Type
Reprint (Version printed in journal)
Authors
Chang, D.
(Kansas State University Manhattan 66506, United States)
Haynes, J. I. Jr
Brady, J. N.
Consigli, R. A.
Spooner, B. S.
Date Acquired
August 21, 2013
Publication Date
April 1, 1993
Publication Information
Publication: Transactions of the Kansas Academy of Science
Volume: 96
Issue: 2-Jan
ISSN: 0022-8443
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: CA07319
CONTRACT_GRANT: NAGW-2328
CONTRACT_GRANT: NAGW-1197
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Number 93-10
NASA Discipline Cell Biology
Non-NASA Center
NASA Program NSCORT

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