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Synthesis and degradation of nitrate reductase during the cell cycle of Chlorella sorokinianaStudies on the diurnal variations of nitrate reductase (NR) activity during the life cycle of synchronized Chlorella sorokiniana cells grown with a 7:5 light-dark cycle showed that the NADH:NR activity, as well as the NR partial activities NADH:cytochrome c reductase and reduced methyl viologen:NR, closely paralleled the appearance and disappearance of NR protein as shown by sodium dodecyl sulfate gel electrophoresis and immunoblots. Results of pulse-labeling experiments with [35S]methionine further confirmed that diurnal variations of the enzyme activities can be entirely accounted for by the concomitant synthesis and degradation of the NR protein.
Document ID
20040112087
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Velasco, P. J.
(University of California Davis 95616, United States)
Tischner, R.
Huffaker, R. C.
Whitaker, J. R.
Date Acquired
August 21, 2013
Publication Date
January 1, 1989
Publication Information
Publication: Plant physiology
Volume: 89
ISSN: 0032-0889
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Life Support Systems

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