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Effect of dinucleoside pyrophosphates on the oligomerization of activated mononucleotides on Na(+)-montmorillonite: reaction of 5'-phosphoro-4-(dimethylamino)pyridinium [4-(CH3)2NpypA] with A5'ppAThe oligomerization of adenosine 5'-phosphoro-4-(dimethylamino)pyridinium (4-(CH3)2-NpypA) and diadenosine 5',5'-pyrophosphate (A5'ppA) (9:1) on Na(+)-montmorillonite was studied. The oligomers were isolated and analyzed by selective enzymatic hydrolyses and the oligomeric composition and the percent of 3',5'-phosphodiester linkages present in each fraction was determined. The longest oligomers formed (11-mers) are slightly shorter than those produced in the absence of A5'ppA (12-mers). Smaller amounts of A5'ppA are incorporated into the oligomers than in the ImpA/A5'ppA reaction. The regioselectivity of 3',5'-phosphodiester bond formation is comparable to that of the oligomerization of 4-(CH3)2NpypA alone. An explanation of these data is proposed and the possible effect of dinucleoside pyrophosphate on prebiotic RNA formation is discussed.
Document ID
20040112192
Acquisition Source
Headquarters
Document Type
Reprint (Version printed in journal)
Authors
Prabahar, K. J.
(Rensselaer Polytechnic Institute Troy, New York 12180-3590, United States)
Ferris, J. P.
Date Acquired
August 21, 2013
Publication Date
December 1, 1997
Publication Information
Publication: Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life
Volume: 27
Issue: 6-May
ISSN: 0169-6149
Subject Category
Exobiology
Funding Number(s)
CONTRACT_GRANT: NAGW-2781
CONTRACT_GRANT: CHE9-619149
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Exobiology

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