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Comparative assessment of the polypeptide profiles from lateral and primary roots of Phaseolus vulgaris LIn Phaseolus vulgaris, primary roots show gravitational sensitivity soon after emerging from the seed. In contrast, lateral roots are agravitropic during early development, and become gravitropic after several cm growth. Primary and lateral root tissues were examined by polyacrylamide gel electrophoresis, coupled with western blotting techniques, to compare proteins which may contribute to the acquisition of gravitational sensitivity. Root tips and zones of cell elongation were compared for each root type, using immunological probes for calmodulin, alpha-actin, alpha-tubulin, and proteins of the plastid envelope. Lateral roots contained qualitatively less calmodulin, and showed a slightly different pattern of actin-related epitope proteins, than did primary root tissues, suggesting that polypeptide differences may contribute to the gravitational sensitivity which these root types express.
Document ID
20040112243
Acquisition Source
Headquarters
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Westberg, J.
(Kansas State University Manhattan 66506-4903, United States)
Odom, W. R.
Guikema, J. A.
Spooner, B. S.
Date Acquired
August 21, 2013
Publication Date
July 1, 1994
Publication Information
Publication: The Journal of experimental zoology
Volume: 269
Issue: 3
ISSN: 0022-104X
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: 9337306-9147
CONTRACT_GRANT: NAGW-1197
CONTRACT_GRANT: NAGW-2328
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Developmental Biology
NASA Discipline Cell Biology
NASA Program NSCORT
NASA Discipline Number 93-10

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