NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Chromosomal instability induced by heavy ion irradiationPURPOSE: To establish the dose-response relationship for the induction of chromosomal instability in GM10115 cells exposed to high-energy iron ions (1 GeV/nucleon, mean LET 146 keV/microm) and gold ions (11 GeV/nucleon, mean LET 1450 keV/microm). Past work has established that sparsely ionizing X-rays can induce a long-lived destabilization of chromosomes in a dose-dependent manner at an incidence of approximately 3% per gray. The present investigation assesses the capacity of High-Z and High-energy (HZE) particles to elicit this same endpoint. MATERIALS AND METHODS: Clonal populations derived from single progenitor cells surviving heavy-ion irradiation were analyzed cytogenetically to identify those clones showing a persistent destablization of chromosomes. RESULTS: Dose-response data, with a particular emphasis at low dose (< 1.0 Gy), indicate a frequency of approximately 4% per gray for the induction of chromosomal instability in clones derived from single progenitor cells surviving exposure to iron ions. The induction of chromosomal instability by gold ions was, however, less responsive to applied dose, as the observed incidence of this phenotype varied from 0 to 10% over 1-8 Gy. Both iron and gold ions gave dose-dependent increases in the yield of chromosomal aberrations (both chromosome- and chromatid-type) measured at the first mitosis following irradiation, as well as shoulderless survival curves having D0=0.87 and 1.1 Gy respectively. CONCLUSIONS: Based on the present dose-response data, the relative biological effectiveness of iron ions is 1.3 for the induction of chromosomal instability, and this indicates that heavy ions are only slightly more efficient than X-rays at eliciting this delayed phenotype.
Document ID
20040112644
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Limoli, C. L.
(University of California San Francisco 94103-0806, United States)
Ponnaiya, B.
Corcoran, J. J.
Giedzinski, E.
Morgan, W. F.
Date Acquired
August 21, 2013
Publication Date
December 1, 2000
Publication Information
Publication: International journal of radiation biology
Volume: 76
Issue: 12
ISSN: 0955-3002
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: CA 73924
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Radiation Health

Available Downloads

There are no available downloads for this record.
No Preview Available