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Cloning and characterization of ftsZ and pyrF from the archaeon Thermoplasma acidophilumTo characterize cytoskeletal components of archaea, the ftsZ gene from Thermoplasma acidophilum was cloned and sequenced. In T. acidophilum ftsZ, which is involved in cell division, was found to be in an operon with the pyrF gene, which encodes orotidine-5'-monophosphate decarboxylase (ODC), an essential enzyme in pyrimidine biosynthesis. Both ftsZ and pyrF from T. acidophilum were expressed in Escherichia coli and formed functional proteins. FtsZ expression in wild-type E. coli resulted in the filamentous phenotype characteristic of ftsZ mutants. T. acidophilum pyrF expression in an E. coli mutant lacking pyrF complemented the mutation and rescued the strain. Sequence alignments of ODCs from archaea, bacteria, and eukarya reveal five conserved regions, two of which have homology to 3-hexulose-6-phosphate synthase (HPS), suggesting a common substrate recognition and binding motif. Copyright 2000 Academic Press.
Document ID
20040141413
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Yaoi, T.
(NASA Ames Research Center Moffett Field CA United States)
Laksanalamai, P.
Jiemjit, A.
Kagawa, H. K.
Alton, T.
Trent, J. D.
Date Acquired
August 22, 2013
Publication Date
September 7, 2000
Publication Information
Publication: Biochemical and biophysical research communications
Volume: 275
Issue: 3
ISSN: 0006-291X
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other

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