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Fas/APO-1 protein is increased in spaceflown lymphocytes (Jurkat)Human lymphocytes flown on the Space Shuttle respond poorly to mitogen stimulation and populations of the lymphoblastoid T cell line, Jurkat, manifest growth arrest, increase in apoptosis and time- and microgravity-dependent increases in the soluble form of the cell death factor, Fas/APO-1 (sFas). The potential role of apoptosis in population dynamics of space-flown lymphocytes has not been investigated previously. We flew Jurkat cells on Space Transportation System (STS)-80 and STS-95 to determine whether apoptosis and the apparent microgravity-related release of sFas are characteristic of lymphocytes in microgravity. The effects of spaceflight and ground-based tests simulating spaceflight experimental conditions, including high cell density and low serum concentration, were assessed. Immunofluorescence microscopy showed increased cell associated Fas in flown cells. Results of STS-80 and STS-95 confirmed increase in apoptosis during spaceflight and the release of sFas as a repeatable, time-dependent and microgravity-related response. Ground-based tests showed that holding cells at 1.5 million/ml in medium containing 2% serum before launch did not increase sFas. Reports of increased Fas in cells of the elderly and the increases in spaceflown cells suggest possible similarities between aging and spaceflight effects on lymphocytes.
Document ID
20040141550
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Cubano, L. A.
(University of Alabama in Huntsville Wilson Hall Room 360, Huntsville, AL 35899, United States)
Lewis, M. L.
Date Acquired
August 22, 2013
Publication Date
May 1, 2000
Publication Information
Publication: Experimental gerontology
Volume: 35
Issue: 3
ISSN: 0531-5565
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
short duration
NASA Experiment Number 9307221
NASA Program Fundamental Space Biology
Non-NASA Center
manned
NASA Discipline Cell Biology
NASA Program Biomedical Research and Countermeasures
STS-80 Shuttle Project
STS-95 Shuttle Project
Flight Experiment

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