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Identification of genes regulated during mechanical load-induced cardiac hypertrophyCardiac hypertrophy is associated with both adaptive and adverse changes in gene expression. To identify genes regulated by pressure overload, we performed suppressive subtractive hybridization between cDNA from the hearts of aortic-banded (7-day) and sham-operated mice. In parallel, we performed a subtraction between an adult and a neonatal heart, for the purpose of comparing different forms of cardiac hypertrophy. Sequencing more than 100 clones led to the identification of an array of functionally known (70%) and unknown genes (30%) that are upregulated during cardiac growth. At least nine of those genes were preferentially expressed in both the neonatal and pressure over-load hearts alike. Using Northern blot analysis to investigate whether some of the identified genes were upregulated in the load-independent calcineurin-induced cardiac hypertrophy mouse model, revealed its incomplete similarity with the former models of cardiac growth. Copyright 2000 Academic Press.
Document ID
20040141595
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Johnatty, S. E.
(Baylor College of Medicine Houston, TX 77030, United States)
Dyck, J. R.
Michael, L. H.
Olson, E. N.
Abdellatif, M.
Schneider, M.
Date Acquired
August 22, 2013
Publication Date
May 1, 2000
Publication Information
Publication: Journal of molecular and cellular cardiology
Volume: 32
Issue: 5
ISSN: 0022-2828
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: 5 R29 HL57970-02
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Cardiopulmonary
Non-NASA Center

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