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The use of multiple indices of physiological activity to access viability in chlorine disinfected Escherichia coli O157:H7A suite of fluorescent intracellular stains and probes was used, in conjunction with viable plate counts, to assess the effect of chlorine disinfection on membrane potential (rhodamine 123; Rh123 and bis-(1,3-dibutylbarbituric acid) trimethine oxonol; DiBAC4(3)), membrane integrity (LIVE/DEAD BacLight kit), respiratory activity (5-cyano-2,3-ditolyl tetrazolium chloride; CTC) and substrate responsiveness (direct viable counts; DVC) in the commensal pathogen Escherichia coli O157:H7. After a 5 min exposure to the disinfectant, physiological indices were affected in the following order: viable plate counts > substrate responsiveness > membrane potential > respiratory activity > membrane integrity. In situ assessment of physiological activity by examining multiple targets, as demonstrated in this study, permits a more comprehensive determination of the site and extent of injury in bacterial cells following sublethal disinfection with chlorine. This approach to assessing altered bacterial physiology has application in various fields where detection of stressed bacteria is of interest.
Document ID
20040141875
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Lisle, J. T.
(Montana State University, Department of Microbiology Bozeman 59717, United States)
Pyle, B. H.
McFeters, G. A.
Date Acquired
August 22, 2013
Publication Date
July 1, 1999
Publication Information
Publication: Letters in applied microbiology
Volume: 29
Issue: 1
ISSN: 0266-8254
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Environmental Health
Non-NASA Center

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