NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Alterations of p53 in tumorigenic human bronchial epithelial cells correlate with metastatic potentialThe cellular and molecular mechanisms of radiation-induced lung cancer are not known. In the present study, alterations of p53 in tumorigenic human papillomavirus-immortalized human bronchial epithelial (BEP2D) cells induced by a single low dose of either alpha-particles or 1 GeV/nucleon (56)Fe were analyzed by PCR-single-stranded conformation polymorphism (SSCP) coupled with sequencing analysis and immunoprecipitation assay. A total of nine primary and four secondary tumor cell lines, three of which were metastatic, together with the parental BEP2D and primary human bronchial epithelial (NHBE) cells were studied. The immunoprecipitation assay showed overexpression of mutant p53 proteins in all the tumor lines but not in NHBE and BEP2D cells. PCR-SSCP and sequencing analysis found band shifts and gene mutations in all four of the secondary tumors. A G-->T transversion in codon 139 in exon 5 that replaced Lys with Asn was detected in two tumor lines. One mutation each, involving a G-->T transversion in codon 215 in exon 6 (Ser-->lle) and a G-->A transition in codon 373 in exon 8 (Arg-->His), was identified in the remaining two secondary tumors. These results suggest that p53 alterations correlate with tumorigenesis in the BEP2D cell model and that mutations in the p53 gene may be indicative of metastatic potential.
Document ID
20040141880
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Piao, C. Q.
(College of Physicians and Surgeons of Columbia University 630 West 168th Street, New York, NY 10032, United States)
Willey, J. C.
Hei, T. K.
Hall, E. J.
Date Acquired
August 22, 2013
Publication Date
August 1, 1999
Publication Information
Publication: Carcinogenesis
Volume: 20
Issue: 8
ISSN: 0143-3334
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: CA 94062
CONTRACT_GRANT: ES 07890
CONTRACT_GRANT: RR11623
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Radiation Health

Available Downloads

There are no available downloads for this record.
No Preview Available