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DNA-directed mutations. Leading and lagging strand specificityThe fidelity of replication has evolved to reproduce B-form DNA accurately, while allowing a low frequency of mutation. The fidelity of replication can be compromised, however, by defined order sequence DNA (dosDNA) that can adopt unusual or non B-DNA conformations. These alternative DNA conformations, including hairpins, cruciforms, triplex DNAs, and slipped-strand structures, may affect enzyme-template interactions that potentially lead to mutations. To analyze the effect of dosDNA elements on spontaneous mutagenesis, various mutational inserts containing inverted repeats or direct repeats were cloned in a plasmid containing a unidirectional origin of replication and a selectable marker for the mutation. This system allows for analysis of mutational events that are specific for the leading or lagging strands during DNA replication in Escherichia coli. Deletions between direct repeats, involving misalignment stabilized by DNA secondary structure, occurred preferentially on the lagging strand. Intermolecular strand switch events, correcting quasipalindromes to perfect inverted repeats, occurred preferentially during replication of the leading strand.
Document ID
20040141893
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Sinden, R. R.
(Texas A&M University Houston 77030-3303, United States)
Hashem, V. I.
Rosche, W. A.
Date Acquired
August 22, 2013
Publication Date
May 18, 1999
Publication Information
Publication: Annals of the New York Academy of Sciences
Volume: 870
ISSN: 0077-8923
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Radiation Health
Review, Tutorial
Non-NASA Center
Review

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