NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Distribution of Candida albicans genotypes among family membersThirty-three families (71 subjects) were screened for the presence of Candida albicans in mouthwash or stool specimens; 12 families (28 subjects) were culture-positive for this yeast. An enrichment procedure provided a twofold increase in the recovery of C. albicans from mouthwash specimens. Nine of the twelve culture-positive families had two positive members each, two families had three positive members each, and one family had four positive members. Genetic profiles were obtained by three methods: pulsed-field gel electrophoresis; restriction endonuclease analysis, and random amplification of polymorphic DNA analysis. DNA fingerprinting of C. albicans isolated from one body site three consecutive times revealed that each of the 12 families carried a distinct genotype. No two families shared the same strain, and two or more members of a family commonly shared the same strain. Intrafamily genotypic identity (i.e., each member within the family harbored the same strain) was demonstrated in six families. Genotypes of isolates from husband and wife differed from one another in five families. All three methods were satisfactory in determining genotypes; however, we concluded that restriction endonuclease analysis provided adequate resolving power.
Document ID
20040141949
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Mehta, S. K.
(NASA Johnson Space Center Houston TX United States)
Stevens, D. A.
Mishra, S. K.
Feroze, F.
Pierson, D. L.
Date Acquired
August 22, 2013
Publication Date
May 1, 1999
Publication Information
Publication: Diagnostic microbiology and infectious disease
Volume: 34
Issue: 1
ISSN: 0732-8893
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Center JSC
NASA Discipline Environmental Health

Available Downloads

There are no available downloads for this record.
No Preview Available