NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
2-deoxy-D-glucose-induced metabolic stress enhances resistance to Listeria monocytogenes infection in miceExposure to different forms of psychological and physiological stress can elicit a host stress response, which alters normal parameters of neuroendocrine homeostasis. The present study evaluated the influence of the metabolic stressor 2-deoxy-D-glucose (2-DG; a glucose analog, which when administered to rodents, induces acute periods of metabolic stress) on the capacity of mice to resist infection with the facultative intracellular bacterial pathogen Listeria monocytogenes. Female BDF1 mice were injected with 2-DG (500 mg/kg b. wt.) once every 48 h prior to, concurrent with, or after the onset of a sublethal dose of virulent L. monocytogenes. Kinetics of bacterial growth in mice were not altered if 2-DG was applied concurrently or after the start of the infection. In contrast, mice exposed to 2-DG prior to infection demonstrated an enhanced resistance to the listeria challenge. The enhanced bacterial clearance in vivo could not be explained by 2-DG exerting a toxic effect on the listeria, based on the results of two experiments. First, 2-DG did not inhibit listeria replication in trypticase soy broth. Second, replication of L. monocytogenes was not inhibited in bone marrow-derived macrophage cultures exposed to 2-DG. Production of neopterin and lysozyme, indicators of macrophage activation, were enhanced following exposure to 2-DG, which correlated with the increased resistance to L. monocytogenes. These results support the contention that the host response to 2-DG-induced metabolic stress can influence the capacity of the immune system to resist infection by certain classes of microbial pathogens.
Document ID
20040142122
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Miller, E. S.
(Texas Tech University Health Sciences Center School of Pharmacy Amarillo 79106, United States)
Bates, R. A.
Koebel, D. A.
Fuchs, B. B.
Sonnenfeld, G.
Date Acquired
August 22, 2013
Publication Date
December 1, 1998
Publication Information
Publication: Physiology & behavior
Volume: 65
Issue: 3
ISSN: 0031-9384
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Regulatory Physiology

Available Downloads

There are no available downloads for this record.
No Preview Available