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Isolation and characterization of Arabidopsis mutants defective in the induction of ethylene biosynthesis by cytokininCytokinins elevate ethylene biosynthesis in etiolated Arabidopsis seedlings via a post-transcriptional modification of one isoform of the key biosynthetic enzyme ACC synthase. In order to begin to dissect the signaling events leading from cytokinin perception to this modification, we have isolated a series of mutants that lack the ethylene-mediated triple response in the presence of cytokinin due to their failure to increase ethylene biosynthesis. Analysis of genetic complementation and mapping revealed that these Cin mutants (cytokinin-insensitive) represent four distinct complementation groups, one of which, cin4, is allelic to the constitutive photomorphogenic mutant fus9/cop10. The Cin mutants have subtle effects on the morphology of adult plants. We further characterized the Cin mutants by analyzing ethylene biosynthesis in response to various other inducers and in adult tissues, as well as by assaying additional cytokinin responses. The cin3 mutant did not disrupt ethylene biosynthesis under any other conditions, nor did it disrupt any other cytokinin responses. Only cin2 disrupted ethylene biosynthesis in multiple circumstances. cin1 and cin2 made less anthocyanin in response to cytokinin. cin1 also displayed reduced shoot initiation in tissue culture in response to cytokinin, suggesting that it affects a cytokinin signaling element.
Document ID
20040172687
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Vogel, J. P.
(University of Illinois at Chicago Chicago, Illinois 60607, United States)
Schuerman, P.
Woeste, K.
Brandstatter, I.
Kieber, J. J.
Evans, M. L.
Date Acquired
August 22, 2013
Publication Date
May 1, 1998
Publication Information
Publication: Genetics
Volume: 149
Issue: 1
ISSN: 0016-6731
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Plant Biology

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