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Information transfer from DNA to peptide nucleic acids by template-directed synthesesPeptide nucleic acids (PNAs) are analogs of nucleic acids in which the ribose-phosphate backbone is replaced by a backbone held together by amide bonds. PNAs are interesting as models of alternative genetic systems because they form potentially informational base paired helical structures. Oligocytidylates have been shown to act as templates for formation of longer oligomers of G from PNA G2 dimers. In this paper we show that information can be transferred from DNA to PNA. DNA C4T2C4 is an efficient template for synthesis of PNA G4A2G4 using G2 and A2 units as substrates. The corresponding synthesis of PNA G4C2G4 on DNA C4G2C4 is less efficient. Incorporation of PNA T2 into PNA products on DNA C4A2C4 is the least efficient of the three reactions. These results, obtained using PNA dimers as substrates, parallel those obtained using monomeric activated nucleotides.
Document ID
20040172811
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Schmidt, J. G.
(The Salk Institute for Biological Studies PO Box 85800, San Diego, CA 92186, United States)
Christensen, L.
Nielsen, P. E.
Orgel, L. E.
Bada, J. L.
Date Acquired
August 22, 2013
Publication Date
December 1, 1997
Publication Information
Publication: Nucleic acids research
Volume: 25
Issue: 23
ISSN: 0305-1048
Subject Category
Exobiology
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Exobiology
Non-NASA Center

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