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Initiation of oncogenic transformation in human mammary epithelial cells by charged particlesExperimental studies have shown that high linear-energy transfer (LET) charged particles can be more effective than x-rays and gamma-rays in inducing oncogenic transformation in cultured cells and tumors in animals. Based on these results, experiments were designed and performed with an immortal human mammary epithelial cell line (H184B5), and several clones transformed by heavy ions were obtained. Cell fusion experiments were subsequently done, and results indicate that the transforming gene(s) is recessive. Chromosome analysis with fluorescence in situ hybridization (FISH) techniques also showed additional translocations in transformed human mammary epithelial cells. In addition, studies with these cell lines indicate that heavy ions can effectively induce deletion, break, and dicentrics. Deletion of tumor suppressor gene(s) and/or formation of translocation through DNA double strand breaks is a likely mechanism for the initiation of oncogenic transformation in human mammary epithelial cells.
Document ID
20040172901
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Yang, T. C.
(NASA Johnson Space Center Houston, TX United States)
Georgy, K. A.
Craise, L. M.
Durante, M.
Date Acquired
August 22, 2013
Publication Date
January 1, 1997
Publication Information
Publication: Radiation oncology investigations
Volume: 5
Issue: 3
ISSN: 1065-7541
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Radiation Health
NASA Center JSC

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