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Composition of the carbohydrate granules of the cyanobacterium, Cyanothece sp. strain ATCC 51142Cyanothece sp. strain ATCC 51142 is an aerobic, unicellular, diazotrophic cyanobacterium that temporally separates O2-sensitive N2 fixation from oxygenic photosynthesis. The energy and reducing power needed for N2 fixation appears to be generated by an active respiratory apparatus that utilizes the contents of large interthylakoidal carbohydrate granules. We report here on the carbohydrate and protein composition of the granules of Cyanothece sp. strain ATCC 51142. The carbohydrate component is a glucose homopolymer with branches every nine residues and is chemically identical to glycogen. Granule-associated protein fractions showed temporal changes in the number of proteins and their abundance during the metabolic oscillations observed under diazotrophic conditions. There also were temporal changes in the protein pattern of the granule-depleted supernatant fractions from diazotrophic cultures. None of the granule-associated proteins crossreacted with antisera directed against several glycogen-metabolizing enzymes or nitrogenase, although these proteins were tentatively identified in supernatant fractions. It is suggested that the granule-associated proteins are structural proteins required to maintain a complex granule architecture.
Document ID
20040173029
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Schneegurt, M. A.
(NASA Headquarters Washington, DC United States)
Sherman, D. M.
Sherman, L. A.
Mitchell, C. A.
Date Acquired
August 22, 2013
Publication Date
February 1, 1997
Publication Information
Publication: Archives of microbiology
Volume: 167
Issue: 2-3
ISSN: 0302-8933
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Cell Biology
NASA Discipline Number 93-10
Non-NASA Center
NASA Program NSCORT

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