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The effect of space flight on monoclonal antibody synthesis in a hybridoma mouse cell lineThe hybridoma cell line, 3G10G5, producing a monoclonal antibody to the major capsid protein VP1 from the avian polyomavirus budgerigar fledgling disease virus, was produced from a Balb/C mouse. This cell line was used to test the effects of microgravity on cellular processes, specifically protein synthesis. A time course study utilizing incorporation of [35S]methionine into newly synthesized monoclonal antibody was performed on STS-77. After 5.5 days, it was observed that cell counts for the samples exposed to microgravity were lower than those of ground-based samples. However, radiolabel incorporation of the synthesized monoclonal antibody was similar in both orbiter and ground control samples. Overall, microgravity does not seem to have an effect on this cell line's ability to synthesize IgG protein.
Document ID
20040173042
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Smiley, S. A.
(Kansas State University Manhattan 66506, United States)
Gillock, E. T.
Black, M. C.
Consigli, R. A.
Spooner, B. S.
Date Acquired
August 22, 2013
Publication Date
February 1, 1997
Publication Information
Publication: Experimental cell research
Volume: 230
Issue: 2
ISSN: 0014-4827
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: CA09418
CONTRACT_GRANT: CA07319
Distribution Limits
Public
Copyright
Other
Keywords
manned
NASA Program Flight
STS-77 Shuttle Project
NASA Program NSCORT
NASA Discipline Cell Biology
Non-NASA Center
NASA Discipline Number 93-10
NASA Discipline Number 00-00
Flight Experiment
short duration

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