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A low, adaptive dose of gamma-rays reduced the number and altered the spectrum of S1- mutants in human-hamster hybrid AL cellsWe examined the effects of a low, adaptive dose of 137Cs-gamma-irradiation (0.04 Gy) on the number and kinds of mutants induced in AL human-hamster hybrid cells by a later challenge dose of 4 Gy. The yield of S1- mutants was significantly less (by 53%) after exposure to both the adaptive and challenge doses compared to the challenge dose alone. The yield of hprt- mutants was similarly decreased. Incubation with cycloheximide (CX) or 3-aminobenzamide largely negated the decrease in mutant yield. The adaptive dose did not perturb the cell cycle, was not cytotoxic, and did not of itself increase the mutant yield above background. The adaptive dose did, however, alter the spectrum of S1- mutants from populations exposed only to the adaptive dose, as well as affecting the spectrum of S1- mutants generated by the challenge dose. The major change in both cases was a significant increase in the proportion of complex mutations compared to small mutations and simple deletions.
Document ID
20040173099
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Ueno, A. M.
(Colorado State University Fort Collins 80523, United States)
Vannais, D. B.
Gustafson, D. L.
Wong, J. C.
Waldren, C. A.
Date Acquired
August 22, 2013
Publication Date
November 4, 1996
Publication Information
Publication: Mutation research
Volume: 358
Issue: 2
ISSN: 0027-5107
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: CA36447
CONTRACT_GRANT: CA56392
CONTRACT_GRANT: CA09236
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Radiation Health

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