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Interkinetic and migratory behavior of a cohort of neocortical neurons arising in the early embryonic murine cerebral wallNeocortical neuronogenesis occurs in the pseudostratified ventricular epithelium (PVE) where nuclei of proliferative cells undergo interkinetic nuclear movement. A fraction of daughter cells exits the cell cycle as neurons (the quiescent, or Q, fraction), whereas a complementary fraction remains in the cell cycle (the proliferative, or P, fraction). By means of sequential thymidine and bromodeoxyuridine injections in mouse on embryonic day 14, we have monitored the proliferative and post-mitotic migratory behaviors of 1 and 2 hr cohorts of PVE cells defined by the injection protocols. Soon after mitosis, the Q fraction partitions into a rapidly exiting (up to 50 microns/hr) subpopulation (Qr) and a more slowly exiting (6 microns/hr) subpopulation (Qs). Qr and Qs are separated as two distributions on exit from the ventricular zone with an interpeak distance of approximately 40 microns. Cells in Qr and Qs migrate through the intermediate zone with no significant change in the interpeak distance, suggesting that they migrate at approximately the same velocities. The rate of migration increases with ascent through the intermediate zone (average 2-6.4 microns/hr) slowing only transiently on entry into the developing cortex. Within the cortex, Qr and Qs merge to form a single distribution most concentrated over layer V.
Document ID
20040173145
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Takahashi, T.
(Massachusetts General Hospital, Harvard Medical School Boston 02114, United States)
Nowakowski, R. S.
Caviness, V. S. Jr
Date Acquired
August 22, 2013
Publication Date
September 15, 1996
Publication Information
Publication: The Journal of neuroscience : the official journal of the Society for Neuroscience
Volume: 16
Issue: 18
ISSN: 0270-6474
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NS28061
CONTRACT_GRANT: NS12005
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Neuroscience
Non-NASA Center

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