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Mechanical load induces sarcoplasmic wounding and FGF release in differentiated human skeletal muscle culturesThe transduction mechanism (or mechanisms) responsible for converting a mechanical load into a skeletal muscle growth response are unclear. In this study we have used a mechanically active tissue culture model of differentiated human skeletal muscle cells to investigate the relationship between mechanical load, sarcolemma wounding, fibroblast growth factor release, and skeletal muscle cell growth. Using the Flexcell Strain Unit we demonstrate that as mechanical load increases, so too does the amount of sarcolemma wounding. A similar relationship was also observed between the level of mechanical load inflicted on the cells and the amount of bFGF (FGF2) released into the surrounding medium. In addition, we demonstrate that the muscle cell growth response induced by chronic mechanical loading in culture can be inhibited by the presence of an antibody capable of neutralizing the biological activity of FGF. This study provides direct evidence that mechanically induced, sarcolemma wound-mediated FGF release is an important autocrine mechanism for transducing the stimulus of mechanical load into a skeletal muscle growth response.
Document ID
20040173264
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Clarke, M. S.
(NASA Johnson Space Center Houston, TX United States)
Feeback, D. L.
Date Acquired
August 22, 2013
Publication Date
March 1, 1996
Publication Information
Publication: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Volume: 10
Issue: 4
ISSN: 0892-6638
Subject Category
Aerospace Medicine
Distribution Limits
Public
Copyright
Other
Keywords
NASA Center JSC
NASA Program Space Physiology and Countermeasures
NASA Discipline Number 26-10
NASA Discipline Musculoskeletal

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