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Comparison of DNA fragmentation and color thresholding for objective quantitation of apoptotic cellsApoptosis is a process of cell death characterized by distinctive morphological changes and fragmentation of cellular DNA. Using video imaging and color thresholding techniques, we objectively quantitated the number of cultured CD4+ T-lymphoblastoid cells (HUT78 cells, RH9 subclone) displaying morphological signs of apoptosis before and after exposure to gamma-irradiation. The numbers of apoptotic cells measured by objective video imaging techniques were compared to numbers of apoptotic cells measured in the same samples by sensitive apoptotic assays that quantitate DNA fragmentation. DNA fragmentation assays gave consistently higher values compared with the video imaging assays that measured morphological changes associated with apoptosis. These results suggest that substantial DNA fragmentation can precede or occur in the absence of the morphological changes which are associated with apoptosis in gamma-irradiated RH9 cells.
Document ID
20050000156
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Plymale, D. R.
(Tulane University School of Medicine New Orleans, LA 70112-2699, United States)
Ng Tang, D. S.
Fermin, C. D.
Lewis, D. E.
Martin, D. S.
Garry, R. F.
Date Acquired
August 22, 2013
Publication Date
September 1, 1995
Publication Information
Publication: Scanning microscopy
Volume: 9
Issue: 3
ISSN: 0891-7035
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: DE10862
CONTRACT_GRANT: AI43754
CONTRACT_GRANT: AI36211
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Neuroscience

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