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Binding and activation of major histocompatibility complex class II-deficient macrophages by staphylococcal exotoxinsMacrophages from C2D transgenic mice deficient in the expression of major histocompatibility complex (MHC) class II proteins were used to identify binding sites for superantigens distinct from the MHC class II molecule. Iodinated staphylococcal enterotoxins A and B (SEA and SEB) and exfoliative toxins A and B (ETA and ETB) bound to C2D macrophages in a concentration-dependent and competitive manner. All four toxins increased F-actin concentration within 30 s of their addition to C2D macrophages, indicating that signal transduction occurred in response to toxin in the absence of class II MHC. Furthermore, ETA, ETB, SEA, and, to a lesser extent, SEB induced C2D macrophages to produce interleukin 6. Several molecular species on C2D macrophages with molecular masses of 140, 97, 61, 52, 43, and 37 kDa bound SEA in immunoprecipitation experiments. These data indicate the presence of novel, functionally active toxin binding sites on murine macrophages distinct from MHC class II molecules.
Document ID
20050000306
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Beharka, A. A.
(Kansas State University Manhattan 66506-4901)
Armstrong, J. W.
Iandolo, J. J.
Chapes, S. K.
Spooner, B. S.
Date Acquired
August 22, 2013
Publication Date
September 1, 1994
Publication Information
Publication: Infection and immunity
Volume: 62
Issue: 9
ISSN: 0019-9567
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NIAID AI-17474
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Cell Biology

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